X-ray Lab

X-ray Laboratory

The X-ray laboratory component of the Macromolecular Crystallography Laboratory is located in room 2750 of the Stephenson Life Science Research Center on the University of Oklahoma-Norman South Research campus. The mission of the laboratory is to help researchers at the university and through out the state of Oklahoma collect X-ray diffraction data on single crystals of protein and/or nucleic acid samples of interest, determine the structure of the molecule of interest and to be able to use this information to further their work.

Services Provided

Applicable fees will be charged for services.

Crystal Screening

Samples can be screened either at room or low temperatures.
Sample characterization includes:
  • determination of unit cell parameters
  • space group determination
  • resolution and overall diffraction quality

  Samples can then either be used for full data collection, stored for future data collection in the X-ray laboratory, at a synchrotron or disposed of.


Equipment for room temperature mounting or cryo preservation can be provided by the lab.


Data Collection

Complete data sets on both native and heavy atom derivatives can be collected. Data sets will stored for a limited time on the laboratory computers. Software is available for the integration and scaling of collected frames.

Structure Solution

Determination and full refinement of the macromolecular sample is available in consultation with the laboratory manager .

Equipment

The Macromolecular Crystallography Laboratory X-Ray facility is equipped with a  Rigaku MicroMax 007HF microfocus X-ray generator.  A set of VariMax HF X-ray optics couple a Dectris Pilatus 200K silicon pixel detector to the generator.  The detector is mounted on a AFC11  4-axis  goniometer.  An Oxford CryosystemsCryostream 700 Series is utilized for low temperature data collection.  The other available X-ray port on the generator has a Rigaku RAXIS IV++ image plate detector coupled to the 007HF with VariMax HR X-ray optics.  An additional OxfordCryosystems Cryostream 700 Series low temperature system is also mounted to the system.  Data collection and processing is done on Linux workstations using HKL3000R from HKL Research, Inc..   LN2 dewars are available for long term crystal storage.

Training

  All interested users must complete the required University of Oklahoma Radiation Safety training before being allowed to work in the X-ray lab.  Users must then complete basic safety training given by the facility manager. Further one on one training on the X-ray system will then take place before the user is allowed to use the instrument unsupervised.