The following instrumentation is available in the PPC Facility on a first-come, first-served basis to trained users.
The PPC Facility has three separate FPLC units for high resolution protein separations and purifications using custom protocols.
We currently have four separate incubator/shakers available for use with general cellular growth and protein expression.
The shakers utilize a common universal orbital platform and identical flask clamps. As such, use is limited to available space and clamp types on any given date.
We provide a high pressure homogenizer unit for bacterial and yeast cell lysis. The unit can reach pressures in excess of 30,000 psi, making it appropriate for all cell types.
We currently use a fixed angle, large format floor centrifuge for all necessary separation steps during purification.
A large, Class II biologucal safety cabinet is utilized for cell culture procedures.
A CO2 incubator is available in the PPC Facility.
The Facility has an LED inverted microscope available for visualization purposes.
For insect cell cultures, we utilize a separate refrigerated incubator containing cell-spin containers.
The Facility provides customers with a full-featured imaging unit with the ability to visualize samples using white light, UV light, chemiluminescence, and fluorescence.
The Facility recently acquired a pUNk dynamic light scattering system for assessing a protein's hydrodynamic size, size distribution, and aggregate population.
The Facility recently acquired a NanoDrop spectrophotometer which can quantify protein and nucleic acid samples and obtain full spectral data using only 1-2 ul of a sample. A cuvette position is also available.
Please schedule an appropriate time block using the corresponding Skedda Calendar. Or contact the director for further information.
BIO-RAD FPLC Calendar
Emulsiflex-C3 Homogenizer Calendar
Innova 42 Calendar
Innova 42R Calendar
Innova 43 Calendar
Innova 43R Calendar
Avanti Centrifuge Calendar
Insect Cell Incubator Calendar
SLSRC 2590 | Norman, OK 73069 | 405-325-1529